高效液相色谱法测定蓝莓干中的原花青素含量

摘要

To evaluate the quality of the products,the HPLC method for the determination of the content of origin proanthocyanidin in blueberry was established. HPLC chromatographic conditions:Diamonsil C18 (250 mm × 4.6 mm,5μm)chromatographic column,flow phase:methanol∶acetonitrile∶water=75∶20∶5;flow rate was 1 mL/min,detection wavelength at 280 nm. The method had good linear relation,Y=5 756.85X+2 715 (0-100 μg/mL),R2=0.999 5. The repeatability and precision were good,and the recovery rate was 99.83%. RSD=0.204 0%.%建立高效液相色谱测定蓝莓干中的原花青素的含量，用于评价产品的优劣。色谱条件：DiamonsilC18（250 mm×4.6 mm，5μm）色谱柱；流动相为甲醇、乙腈与水，比例为75∶20∶5；流速：1 mL/min；检测波长为280 nm。该方法对原花青素具有良好的线性，Y=5756.85X+2715（0～100μg/mL），R2=0.9995；重复性和精密度良好，样品加标回收率在99.83%。RSD=0.2040%。