碱溶酸沉法提取黑木耳蛋白质研究

摘要

In order to optimize the extraction process of protein from Auricularia auricular,solid-liquid ratio, extraction time,temperature and alkali concentration were investigated for their effects. The optimal extraction conditions were explored by response surface methodology. The results showed that optimal protein extraction parameters were temperature 50℃,solid-liquid ratio 1∶90 (g/mL),extraction time 2.5 h,alkali concentration 0.07mol/L. Under these conditions,the yield of protein from Auricularia auricular was 4.52%. The results indi-cated that this protein extraction method was distinctly effective when applied on Auricularia auricular.%为优化黑木耳蛋白质的碱溶酸沉法提取工艺，考察料液比、提取时间、提取温度、碱液浓度对黑木耳蛋白质得率的影响。采用响应面法优化黑木耳蛋白质提取的工艺条件。结果表明：黑木耳蛋白质的最佳提取工艺参数为：提取温度50℃、料液比1∶90（g/mL）、提取时间2.5 h、碱液浓度0.07 mol/L。在此最佳条件下，黑木耳蛋白质的得率为4.52%。由此可知，碱溶酸沉法可以较好地提取黑木耳蛋白质。