改良胰蛋白胨大豆肉汤培养基的应用效果

摘要

评估GB 4789.11-2014《食品安全国家标准食品微生物检验β型溶血性链球菌检验》中改良胰蛋白胨大豆肉汤培养基的质量。选取国内三家（标记为A、B、C）和国外一家（标记为D）培养基生产商的胰蛋白胨大豆肉汤培养基（商品化脱水合成培养基）和相应添加剂，使用目标菌（乙型溶血性链球菌FSCC225016）和非目标菌（大肠埃希氏菌ATCC25922），依据GB4789.28-2013《食品安全国家标准食品微生物检验培养基和试剂的质量要求》中选择性增菌培养基的定性测试方法进行质量评估。同时不加添加剂进行试验。国内A、B和C三家改良胰蛋白胨大豆肉汤培养基中目标菌浊度值均为0，非目标菌浊度值为0；未加试剂的培养基目标菌浊度值均为0，非目标菌浊度值为0。国外D改良胰蛋白胨大豆肉汤培养基中目标菌浊度值为2，非目标菌浊度值为0；未加试剂的培养基目标菌浊度值为2，非目标菌浊度值为1。国内A、B、C三家的改良胰蛋白胨大豆肉汤培养基质量评估证明是胰蛋白胨大豆肉汤培养基（不加添加剂）基础成分的问题；国产胰蛋白胨大豆肉汤培养基质量有待提高。%Evaluation of GB 4789.11-2014in modified tryptone soy broth medium quality. a total of three domestic(la-beled A, B, C) and foreign(labeled D) a culture based manufacturer of tryptone soya broth culture base(com-mercial dehydration synthetic culture medium) and the corresponding additives, use target bacteria (β-hemolytic streptococcus ATCC21059) and non target bacteria (Escherichia coli ATCC25922,Staphylococcus aureus ATCC25923), according to the GB4789.28-2013in selective enrichment culture based qualitative test method for quality evaluation. At the same time without additives test. The A, B and C three modified tryptone soy broth culture medium target bacteria turbidity values are 0 and non target bacteria turbidity value of 0;with-out reagent medium target bacteria turbidity values are 0, non target bacteria turbidity value of 0. Foreign D im-provement of the pancreatic tryptone soya broth culture medium target bacteria turbidity value is 2, non target bacteria turbidity value of 0;without reagent culture based target bacteria turbidity value of 2, non target bacte-ria turbidity values for 1. Domestic A, B, C three modified tryptone soy broth medium quality assessment that is tryptone soya broth culture medium (without additives) based problem;domestic tryptone soya broth culture medium quality needs to be improved.