HS-GC-MS法和SDE-GC-MS法分析杜氏盐藻挥发性成分

摘要

The volatile components of Dunaliella Salina were isolated by headspace(HS)injection and simultaneous distillation extraction(SDE)methods and then identified by gas chromatography-mass spectrometry(GC-MS).The result showed that 39 volatile components were identified,including alkanes(8), alkenes(2),arenes(3),alcohols(5),ketones(9),aldehydes(6),esters(4),phenol(1)and furan(1).Among them, 19 kinds of compounds were identified from HS injection, and the main volatile compounds were 8-heptadecene(20.18 %), 3-methyl-1-butanol acetate(17.25 %), 3-methyl-1-butanol(7.18 %)and heptadecane(6.21 %).29 kinds of compounds were identified from SDE method,and the main compounds were dihydroactinidiolide(27.50 %),8-heptadecene(23.37 %),heptadecane(6.71 %)and o-xylene(4.93 %). Combined with the two methods could be achieved more comprehensive analysis of volatile components for Dunaliella salina.%采用顶空进样法(headspace,HS)和同时蒸馏萃取法(simultaneous distillation extraction,SDE)对杜氏盐藻的挥发性成分进行萃取,经气相色谱质谱仪分离和检测.共检测出39种挥发性成分.包括烷烃类8种、烯烃类2种、芳香烃类3种、醇类5种、酮类9种、醛类6种、酯类4种、酚类1种和呋喃类1种.其中顶空进样法检测出19种,相对含量较高的是8-十七烯(20.18 %)、3-甲基-1-乙酸丁酯(17.25 %)、3-甲基-1-丁醇(7.18 %)和十七烷(6.21 %);同时蒸馏萃取法检测出29种,相对含量较高的是二氢猕猴桃内酯(27.50 %)、8-十七烯(23.37 %)、十七烷(6.71 %)和邻二甲苯(4.93 %).两种方法相互结合,更能对杜氏盐藻挥发性成分进行全面的分析.