高产乙酸乙酯酵母的产酯条件研究

摘要

A higher yield of ethyl acetate yeast Y14 was selected from starter-making for liquor-making, the production volume of total ester was 2.152g/L in corn saccharification mash.the strains was identified as Hansenula anomala.Firstly through the single factor test to study the effects of diffident culture mediums and diffident culture methods on esterproducing, the results showed that the best ester-producing culture medium for Y1 yeast was the wheat saccharification mash, the ester yield of static culture was 40％ higher when compared with shaking culture.Selected several factors to carry on the orthogonal test once more, the result indicated that the optimization plan was as followed: brix was 8°, pH was 5,temperature was 25℃, culture time was 4 days, and ethyl alcohol was 2％, total ester production of Y14 was 4.812g/L in this conditions.%从酿酒酒曲中分离筛选出一株产乙酸乙酯能力较强的酵母菌Y14,在玉米糖化液中总酯产量为2.152g/L.菌株通过VITEK-32微生物鉴定仪鉴定为Hansenula anomala.通过单因素试验,确定了该酵母菌株最佳产酯培养基是小麦糖化液,而且静止培养比振荡培养产酯高40%.再选取温度、糖度、pH值、发酵时间、酒精添加量五因素设计五因素四水平的正交试验,得出该酵母菌株最佳产酯条件为:糖度为8°,pH为5,温度为28℃,培养时间是4d,酒精添加量为2%.在该优化产酯方案下Y14的产酯量为:4.812g/L.