产壳聚糖酶海洋真菌鉴定、寡营养培养基优化及廉价培养基研究

摘要

A chitosanase-producing marine strain was isolated by our laboratory. Identification by rDNA - ITS sequence analysis, and it is Penicillium oxalicum. This is the first time that Penicillium oxalicum＇ s the ability of producing chitosanase is reported. For the purpose of fermentation chitosanase in industrial, we studied oligotrophic medium components, such as source of carbon, nitrogen source, saltresistance, acidresistance and rotary speed. We explored different kinds of cheap medium for production of chitosanase. We found the soybean powder was the best, and the medium of soybean powder was as follow （g/L seawater） ： soybean powder 25 g, and the optimal enzyme activity was 8.5 U/mL. This medium was insufficient 5% of the cost of original. It provided direct theory and experiment foundation on industrialized production of chitosanase.%对筛选出的产壳聚糖酶海洋真菌进行了rDNA—ITS序列分析技术鉴定，确定该菌为Penicillium oxalitun，这是首次报道该菌属具有产壳聚糖酶的能力。该研究旨在为工业化发酵生产壳聚糖酶提供实验指导。实验在已确定的寡营养培养基的基础上，探讨适宜碳源、氮源、耐盐性、耐酸性、摇床转速等发酵因素对产壳聚糖酶的影响，并探索不同种类的廉价培养基发酵产酶。结果显示黄豆粉培养基的产酶效果较为理想，最终确定培养基为（g／L海水）：黄豆粉25g，酶活力最高为8．5U／mL，该培养基成本不足原发酵培养基成本的5％，为