两歧双歧杆菌BB-G90生产工艺的优化

摘要

Mediums and protective agents of Bifidobacterium were screened for maximum viable count, which was the best to obtain maximum viable counts for Bifidobacterium bifidum BB-G90. Growth parameter of B. bifidum BB- G90 were confirmed by fermentation with controlled pH value of 5.0 ± 0.5. Results of the research showed that medi- um No. 3 was the best and the most appropriate protective agent of B, bifidum BB-G90 was No, 3. With controlled pH value of 5.0 ± 0.5 the viable cell number of B. bifidum BB-G90 could reach 1.80 ± 109 CFU/mL in the optimal growth medium at 37℃ for 22 h under anaerobic condition. Living bacteria content of B. bifidum BB-G90 powder were 4.10 ± 10n CFU/g after freeze-drying.%对两歧双歧杆菌的4种发酵培养基进行了筛选，确定了适合两歧双歧杆菌BB—G90生长的发酵培养基；研究了5种冻干保护剂对两歧双歧杆菌BB—G90活菌的影响。利用筛选的发酵培养基培养BB—G90，在调控pH=5．04-0．5条件下进行300L罐中试发酵试验，确定了发酵参数及冻干保护剂配方。试验结果表明：两歧双歧杆菌BB—G90在优化的发酵培养基、适宜的冻干保护剂及调控pH=5．0±0．5条件下发酵终止时间为22h，此时，发酵液OD^600值为5．62，发酵液活菌数为1．80×10’CFU／mL，发酵液经离心、乳化及冻干后，菌粉活菌数为4．10×10^11CFU／g。