应用高效复合培养基M3D与紫外诱变法筛选高效发酵啤酒酵母菌株

摘要

The high efficient composite medium M3D combined with ultraviolet mutagenesis was used to select beer yeast with improving multiple brewing properties such as fast sugar descending speed,low diacetyl,and low ethanal.6 yeast strains(M3D03,M3D05M3D08,M3D19,and M3D22) showed significantly improved brewing properties compared with control.The multiple positive mutation rate reached 24.00％.The practice application effect of this method was verified by the qualified sensory evaluation and 100 L pilot scale test of M3D03 strain showing similar result as experimental brewing test.%采用高效复合培养基M3D将高降糖速度、低双乙酰、低乙醛3项啤酒酵母发酵特性的选育方法进行同步综合,结合紫外诱变法可筛选出同时具备3种发酵特性的啤酒酵母菌株,从而对出发菌株的发酵性能进行综合提升.对应用该法筛选得到的实验菌株进行低温发酵,得到6株(M3D03、M3D05、M3D08、M3D11、M3D19、M3D22)相对出发菌株KB综合发酵性能显著提升的优良菌株,综合正突变率达到24.00％.菌株M3 D03的100L中试试验结果与实验室规模低温发酵各发酵指标变化率相差不大,且所酿啤酒风味良好.