Purification of Bovine Interphotoreceptor Retinoidbinding Protein and Its Uveitogenicity in Lewis Rats

摘要

Interphotoreceptor retinoid-binding protein, IRBP, not only functioning as a shuttle to carry the retinoid between photoreceptor cells and pigment epithelium, but also inducing experimental autoimmune uveoretinitis (EAU), was purified by ConA Sepharose affinity chromatography from the fractions containing IRBP obtained in the course of ion-exchange chromatography by which the bovine retinal S-antigen was purified. This much simplified method allows more rapid purification of the two kinds of protein. EA...