理化因素及感染复数对噬菌体MS2活性的影响

摘要

As an indicator of potential enterovirus,bacteriophage MS2 has become one of the most important research directions about the microbiological safety evaluation system for recycled wastewater.In this paper,survival rate of bacteriophage MS2 is determined in solutions with different temperature,pH,ultraviolet rays and infection multiplicity.The results showed that it can be inactivated completely when heating at 70℃ heating for 5 minutes.MS2 had relatively high stability at pH value of 4~10.MS2 was inactivated completely under ultraviolet rays for 25 minutes.The optimal multiplicity of infection was 1.%噬菌体MS2作为潜在的水中肠道病毒指示生物,已逐渐成为回用水微生物学安全评价指标的主要研究方向之一。测定了温度、pH值、紫外线和感染复数对噬菌体MS2活性的影响。结果表明：噬菌体MS2在70℃时5 min内即会全部失活,在pH值4～10的范围内均可稳定存在,在短波紫外线照射下25 min内即可失活;噬菌体MS2感染其宿主大肠杆菌的最佳感染复数为1。