Toluene-degradating strains were isolated from petroleum-contaminated soil of Dagang oilfield. Eight bacterium were screened with the method of enrichment and dilution, which were identified as Bacillus aquimaris, Enterobacter, Pseudomonas putida ,Microbacterium,Arthrobacter,Microbacterium,Staphylococcu,Planococcus rifietoensis by 16S rDNA I-dentification.and named as TD1-TD8 respectively. Among the eight bacteria,TD3 presented best performance for toluene degradation,and the max degradation rate was 96. 3%. The optimum growth conditions were pH = 7 and 30 C of temperature. TD3 were added in the activated sludge of the Hangu chemical wastewater treatment plant for bioagmentation degradation of toluene,the biodegradation rate of toluene was increased from 86. 0% to 94. 5%.%从大港油田土壤中分离降解甲苯的微生物,得到8株优势菌,分别命名为TD1至TD8.经16S rDNA鉴定分别为芽孢杆菌属(Bacillus aquimaris)、肠杆菌属(Enterobacter)、假单孢菌属(Pseudomonas putida)、微杆菌属(Microbacterium)、节杆菌属(Arthrobacter)、微杆菌属(Microbacterium)、葡萄球菌属(Staphylococcus)和动性球菌属(Planococcus ri fietoensis).其中TD4和TD6都属于微杆菌属,TD3降解甲苯能力最强,甲苯降解率达到96.3％.通过实验得到TD3降解甲苯最佳条件.将TD3加入到含甲苯的汉沽化工园区化工废水活性污泥中,甲苯降解率由原来的86.0％提高到94.5％.
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