Objective:To establish an HPLC method of determining 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Pushen Cap-sules.Methods:Agilent C18 column(4.6 mm ×250 mm, 5 μm) was used,the mobile phase was acetonitrile-water (17∶83) and the detection wavelength was 320 nm.Results:The linear range of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside was 0.0320-0.960 6 μg( r=1.000 0) ,the average recovery was 96.6%with RSD 1.3%( n=6).Conclusion:The method is reliable,accurate and can be used to the quality control of the preparation.%目的:建立蒲参胶囊中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC测定方法。方法:采用Agilent C18柱(4.6 mm ×250 mm,5μm),流动相为乙腈-水(17∶83),检测波长为320 nm。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0320~0.9606μg范围内呈线性关系(r=1.0000),平均回收率为96.6%(n=6),RSD为1.3%。结论:所建方法简便、准确,可用于该制剂的质量控制。
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