Immune-related DNA methylation signature associated with APLN expression predicts prognostic of hepatocellular carcinoma

摘要

TIME,Immunity,Prognosis,BioinformaticsThis study used transcriptome and epigenetic data to predict the prognosis of immune-related genes(IRGs)Apelin(APLN)in patients with hepatocellular carcinoma(HCC).The TCGA database has gene expression and clinical data for HCC.And DNA methylation 450 k data for HCC was download from the University of California Santa Cruz(UCSC)Xena browser.Performing clinical and prognostic analysis of APLN expression,results show that APLN is highly expressed in tumor samples.And it has an increasing trend with the development of clinical stage and T stage.To explore the prognostic role of APLN,the Immune-related DNA methylation(DNAm)sites associated with APLN analyzed by bioinformatics.Univariate COX screened the methylation sites that are related to both APLN and survival.The risk score related to methylation site signature was determined according to their least absolute shrinkage and selection operator(LASSO)coefficients.Then the patients were divided into high-risk groups and low-risk groups.Significant differences in overall survival(OS)were found in the training cohort.Nomogram shows that APLN or methylation signature can effectively predict the prognosis of HCC patients.In summary,APLN may be a diagnostic and prognostic marker for HCC.