以种子特异性表达的大豆凝集素基因启动子、终止子以及棉株体内硬脂酰-ACP△9脱氢酶的基因ghSAD-1进行例位重复基因构建,以农杆菌介导法转化陆地棉品种Coker315,获得了较高的转化频率.PCR、Southern杂交及种子内脂肪酸组分含量分析.结果表明,该倒位重复基因的表达及沉默效应在不同的转基因品系间存在差异,硬脂酸含量分别由对照的2%提高至4%～38.2%,而棕榈酸含量则明显降低.高含硬脂酸的转基因品系可作为棉子油品质改良的基础材料,具有极大的潜在利用价值.%A partial inverted repeat based gene construct was produced and cloned into the pBI-Lectin binary vector, using the strong seed specific soybean Lectin promoter and cotton stearyol-ACP △9 desaturase gene ghSAD-1.The cotton cultivar G. hirsutum cv. Coker315was then transformed by the construct through Agrobacteriurn mediated infection. A relatively high transformation frequency was obtained.Further analysis by PCR, Southern blotting and fatty acid composition analysis demonstrated that the expression or silencing effectiveness of the transgene was not in the same level among various transgenic lines, where the stearic acid content was increased from 2 percent of the control to a range of 4 ～ 38. 2 percent. These materials are valuable for the further improvement of current cottonseed oil in the future.
展开▼
