根癌农杆菌介导的真菌遗传转化研究进展

摘要

农杆菌介导遗传转化成为真菌实现菌种改良、新基因标记及目的基因的筛选、分离和克隆的重要方法之一.该方法操作简单、受体范围广、转化效率高、单拷贝率高及突变体遗传稳定,解决了传统转化方法的瓶颈.利用该方法成功实现了多种真菌大容量高质量突变体库的构建,且在各类真菌突变体库构建的基础上,相关基因的筛选和功能基因的验证工作成为下一步的研究热点,进而为各类病原菌致病机理的深入研究提供依据,为抗病育种等亟待解决的问题奠定基础.%Agrobacterium tume/aciens-mediated transformation(ATMT) has become an important tool for strain improvement, new genes' tagging and target gene's screening, isolating and cloning. The advantages of this method are simple operation, a wide host range, highly efficient transformation, and a high frequency of single inserted T-DNA. Mutant libraries of many fungi with high capacity and quality have been constructed by ATMT. The screening and identification of related functional genes will be the following research focus on the basis of these mutant libraries. This research will provide a basis for further studies on pathogenic mechanisms and will aid disease-resistance breeding.