Amino acids application enhances flowers insecticidal protein content in Bt cotton

摘要

Background:Low insecticidal protein expression at reproductive organs affect insect resistance in Bt transgenic cotton.In order to enhance flower insecticidal protein expression,the conventional cultivar Sikang1 (S1) and the hybrid cultivar Sikang3 (S3) were used as experimental materials;the applications of selected 5 types of amino acids and 21 types of amino acids were sprayed on the flowers in 2016 and 2017 cotton growing seasons.Results:The flower Bt protein contents increased significantly under the two amino acid treatments in both cultivars,the Bt protein concentration increased by 15.2 to 25.8％ compared with the control.However,no significant differences were detected between the two treatments of amino acid application.Increased amino acid and soluble protein contents,enhanced GPT,GOT,protease,and peptidase activities were observed under the amino acid application at the flowering stage.Conclusions:These results suggest that exterior application of the amino acids treatments could bolster the flower insecticidal protein expression.