Investigation on phytochemical, antimicrobial activity and essential oil constituents ofNardostachys jatamansi DC. in different regions of Nepal

摘要

Objective:To study chemical constituents of essential oil of the roots ofNardostachys jatamansi found in different regions of Nepal and also to investigate phytochemical as well as antimicrobial activity of the sample with high yield of essential oil. Methods:The essential oils of roots of plant from five different regions were extracted by hydro-distillation and analyzed by gas chromatography-mass spectroscopy for their chemical constituents. The root samples were also subjected to hydro-alcoholic extraction and then fractionated with hexane, chloroform,n-butanol and water so as to perform phytochemical screening and antimicrobial activity. Results:The essential oil yield of sample from Jumla was found to be the highest followed by a sample from Nepalgunj, Surkhet and Kathmandu whereas that of sample from Dharan was found to be the lowest. Gas chromatography-mass spectrometer analysis of essential oil of five samples showed that “2-beta pinene” appeared dominated in three samples, namely, 6VJ Nepalgunj,9VJA Jumla and 10VJ Surkhet. Similarly “alkohol aus neoclovenoxid” in 8VJ Dharan and “methoxy phenyloxime” in13VJA Kathmandu was found to be present in the highest amount. Phytochemical screening of different fractions of sample9VJA Jumla showed the presence of alkaloids, terpenoids, glycosides, proteins and amino acids, and carbohydrates etc. Antimicrobial susceptibility test of same fractions showed then-butanol fraction potent against all pathogens and most affected one wasEscherichia coli. Conclusions: Our study suggests that the essential oil ofNardostachys jatamansi found in Nepal contains more than 80 compounds with their quality and quantity differing from place to place.