目的 探讨小鼠海马中突触囊泡蛋白与铅对小鼠学习记忆功能影响的相关性.方法 将雄性昆明小鼠24只,随机平均分为两组,对照组和铅染毒组,对照组饲以蒸馏水,铅染毒组饲以2.4 mmol/L的醋酸铅水溶液,在暴露后30 d,进行Morris 水迷宫实验以检测其逃逸潜伏期,采用Western blot法检测海马中突触囊泡蛋白水平,并采用HE切片方法观察处理前后小鼠海马脑区病理变化.结果 Morris水迷宫结果显示铅暴露后30 d可导致小鼠逃逸潜伏期延长(P<0.05),且暴露后30 d铅染毒组小鼠海马中突触囊泡蛋白表达显著减少(P<0.05),但脑组织HE切片显示铅暴露30 d后并未引起脑组织海马脑区病理学改变.结论 慢性铅暴露可导致小鼠学习记忆功能损伤,且这种损伤可能与其海马中突触囊泡蛋白表达下降有关.%Objective To observe the effect of chronic lead contamination on the function of learning and memory in mice and the relevance with synaptophysin in hippocampus .Methods 24 mice were divided randomly into experimental group and control group . Mice in experimental group were fed with lead acetate at concentrations (2 .4 mmol/L),and those in control group were fed with distilled water instead .After 30 days exposure,the escape latency was tested by Morris water maze ,the expression levels of synaptophysin in hippocampus were detected by western blot ,and the pathological change of hippocampus in mice was observed by HE staining after treated processing .Results 30 days after lead exposure,escape latency of mice extended (P＜0 .05),and synaptophysin protein expression in hippocampus decreased significantly (P＜0 .05) in experimental group ,compared with control group . No histological alteration was observed in the brains intoxicated with lead exposure .Conclusion Chronic lead exposure can lead to learning and memory impairment in mice ,and the damage may be related with the down-regulation of hippocampal synaptophysin protein expression .
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