目的 探讨β-榄香烯对内皮祖细胞(EPCs)参与肿瘤血管形成的抑制作用.方法 原代培养大鼠骨髓来源内皮祖细胞,胃癌细胞株SGC-7901培养上清液刺激与β-榄香烯干预,四唑氮蓝还原反应(MTT)法检测内皮祖细胞增殖;碘化丙啶(PI)标记流式细胞仪检测细胞周期,Matrigel检测细胞管腔形成能力.结果 β-榄香烯抑制肿瘤细胞上清液诱导的内皮祖细胞的增殖,并呈剂量和时间依赖性.细胞周期检测结果显示,β-榄香烯干预后,G1期细胞增多,进入S期及G2期细胞减少,细胞周期被阻滞于G1期;接种于Matrigel的内皮祖细胞形成管腔数目减少.结论 β-榄香烯可抑制内皮祖细胞的增殖,阻滞其从G1期进入S期,并降低其形成管腔的能力.%Objective To discuss the inhibitory action of β-elemene on endothelial progenitor cells(EPCs) angiogenesis. Methods EPCs were primarily cultured and treated with tumor cells supernatant and β-elemene; MTT assay was used to evaluate the proliferation of EPCs; the cell cycle was analyzed by using flow cytometry with PI staining; Matrigel was used to evaluate the angio-genic ability of EPCs. Results After treating with tumor cells supernatant and β-elemene, the proliferation of EPCs was apparently inhibited in a dose-dependent and time-dependent manner; FCM assays showed that EPCs in G1 phase were increased, while in S phase and G2 phase were decreased, which indicated that G1 phase arrest happened; EPCs after treating with tumor cells supernatant and β-elemene, were planted to Matrigel, and the number of tube formation was decreased notably compared with control group. Conclusion β-elemene can directly influence the proliferation and cell cycle of EPCs, arrest cells entering S phase from G1 phase and decrease their angiogenic ability.
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