凝血酶敏感蛋白1对体外培养大鼠肺动脉平滑肌细胞的影响

摘要

Objective To investigate the effect of thrombospondin 1(TSP-1) on the cell proliferation of cultured rat pulmonary artery smooth cell (PASMCs) in vitro .Methods Rat PASMCs were cultured in vitro ,and treated with different concentrations (10-12 、10-11 、10-10 mol/L) of TSP-1 for 12 ,24 ,48 h .The cell proliferation was quantified by MTT assay .The cell cycle of PASMCs was measured by flow cytometric(FCM) analysis .Results MTT assay showed that TSP-1 promoted the proliferation of PASMCs significantly ,and the effect was concentration-dependent and time-dependent .FCM analysis indicated that TSP-1 increased the percentage of S phase .The percentage of S phase of PASMCs were increased after treated with thrombospondin-1 for 12 h , slight down after 24 h ,while reached a maximal level at 48 h .Conclusion The TSP-1 promotes rat PASMCs proliferation in a con-centration-dependent and time-dependent manner .%目的：了解凝血酶敏感蛋白1（TSP-1）对体外培养的大鼠肺动脉平滑肌细胞（PASMCs）的影响。方法体外培养大鼠PASMCs ，加入不同浓度（10-12、10-11和10-10 mol/L）TSP-1处理12、24、48 h ，采用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）法检测细胞增殖，流式细胞术（FCM）检测细胞周期。结果 TSP-1刺激后，MMT 法实验结果显示 TSP-1显著促进大鼠PASMCs增殖，此作用呈浓度和时间依赖性。FCM分析显示TSP-1使S期细胞比例增加。TSP-1处理12 h后，S期细胞比例增加，24 h时轻微下降，48 h时达高峰。结论 TSP-1呈浓度和时间依赖性地促进大鼠PASMCs增殖。