视网膜母细胞瘤组织SUZ12及MMP-9蛋白的表达及意义研究

摘要

Objective To observe the expression of inhibitor of Zeste 12(SUZ12) and matrix metalloproteinase-9(MMP-9) in retinoblastoma(RB) and their significance .Methods The paraffin embedded specimens from 56 patients with RB were studied ,10 patients belong to intraocular stage ,28 patients intraocular pressure increased stage and 18 patients outside ocular stage among them .22 ,16 ,12 ,and 6 patients with invasion degree(N) from N0-N3 differently ,while10 ,16 ,18 and 12 patients with cell differenti-ations(D) from D0-D3 differently .The expressions of SUZ12 and MMP-9 protein in 56 patient s were detected in different clinical stages ,invasion degree and cell differentiation by immunohistochemical staining and their correlations were analyzed .Results The expressions of SUZ12 and MMP-9 in RB tissues[(31 .48 ± 12 .20)% ,(34 .49 ± 13 .25)% ]were obviously higher than in para-cancer tissue[(0 .26% ± 0 .06)% ,(0 .36 ± 0 .08)% ](P<0 .01) .The expression of SUZ12 was related to clinical stages ,invasion degree and cell differentiation .The expression of SUZ12 and MMP-9 in intraocular pressure increased stage was obviously higher than in-traocular stage(t=10 .299 ,16 .253 ;P<0 .01) ,and outside ocular stage was obviously higher than intraocular stage (t= 20 .819 , 36 .417 ;P<0 .01)and intraocular pressure increased stage (t= 10 .348 ,15 .061 ;P< 0 .01) .The expression of SUZ12 and MMP-9 was increasing from N0 to N3 but decreasing from D0 to D3 .The expression of SUZ12 in RB was related to MMP-9 expression(r=0 .840 ,P=0 .000) .Conclusion The expression of SUZ12 and MMP-9 increased in RB tissue and it is helpful to predict the malig-nancy degree of RB .%目的：探讨Zeste基因抑制子12（SUZ12）及基质金属蛋白-9（MMP-9）在视网膜母细胞瘤（RB）中的表达及意义。方法56例RB石蜡包埋标本作为研究对象，其中眼内生长期10例，眼内压增高期28例，眼外扩展期18例；侵袭程度（N ）由N0～N3分别为22、16、12、6例；细胞分化程度（D）由D0～D3分别为10、16、18、12例。免疫组织化学方法检测SUZ12、MMP-9蛋白在不同临床分期、侵袭程度及分化程度的RB标本中的表达并进行相关性分析。结果 SUZ12及M M P-9蛋白在RB组织中的表达较癌旁组织明显增高（P＜0．01）。SUZ12及MM P-9的表达与RB的临床分期、侵袭程度（N0～N3）及细胞分化程度（D0～D3）相关。眼内压增高期SUZ12及MMP-9蛋白表达均显著高于眼内生长期（t＝10．299，16．253；P＜0．01，P＜0．01）；眼外生长期显著高于眼内生长期（t＝20．819，36．417；P＜0．01，P＜0．01）及眼压增高期（t＝10．348，15．061；P＜0．01，P＜0．05）。SUZ12及MMP-9由N0～N3呈逐渐升高趋势，由D0～D3呈逐渐下降趋势，且SUZ12与MMP-9在RB中的表达相关性显著（r＝0．840）。结论 SUZ12及M M P-9在RB组织中呈高表达，并且可预测RB的恶性程度。