血必净注射液和丹参注射液治疗兔肢体缺血再灌注损伤的比较研究

摘要

目的：研究血必净注射液及丹参注射液对兔肢体缺血再灌注损伤治疗效果和机制的差异。方法30只新西兰大耳兔分为对照组、血必净组和丹参组，每组10只。采用Crinnion等的兔下肢缺血再灌注损伤模型，各组于恢复血流后给予相应治疗；对照组给予生理盐水10 mL ，血必净组和丹参组分别给予4 mL/kg血必净注射液和2 mL/kg丹参注射液，并加生理盐水至10 mL ，静脉输注。再灌注前和再灌注后的1、2、4 h分别采集静脉血测定活化部分凝血酶时间（APTT）、纤维蛋白原（FIB）、国际标准比率（PT-INR）、凝血酶原时间（PT）、清蛋白（ALB）、血清乳酸脱氢酶（LDH）、血清肌酸激酶（CK）、丙二醛（MDA）、超氧化物歧化酶（SOD）。结果血必净组APTT再灌注后1、4 h及丹参组缺血再灌注后4 h较对照组有明显改善（P＜0．05）；血必净组PT缺血再灌注后较缺血再灌注前明显延长（P＜0．05）；血必净组、丹参组缺血再灌注后4 h FIB含量较缺血再灌注前有显著增高（P＜0．05）；血必净组缺血再灌注后LDH、CK含量显著低于对照组（P＜0．05）；血必净组缺血再灌注后2 h及丹参组缺血再灌注后1、2 h MDA含量显著低于对照组（P＜0．05）；血必净组缺血再灌注后1～4 h及丹参组缺血再灌注后2 h SOD活性显著高于对照组（P＜0．05）。结论血必净注射液和丹参注射液均可通过调节机体凝血功能、抗自由基等作用来减轻肢体缺血再灌注损伤；血必净注射液较丹参注射液能更好地减轻肌组织损伤。%Objective To study and compare the effects and mechanisms between the Xuebijing injection and Danshen injection on ischemic-reperfusion injury of rabbit limb .Methods 30 New Zealand rabbits were randomly divided into control group ,Xuebi-jing group and Danshen group ,with 10 in each group .The Crinnion model of ischemic-reperfusion injury of rabbit limb was used . Each group was treated correspondingly after the blood flow was restored .Xuebijing group and Danshen group were treated with 4 mL/kg Xuebijing injection 2 mL/kg Danshen injection in saline with 10 mL total volume .Control group was treated with 10 mL sa-line .Before releasing clip and 1 h ,2 h and 4 h after reperfusion ,the blood samples were collected for detecting clotting function (APTT ,FIB ,PT-INR ,PT) ,biochemistry items (ALB ,LDH ,CK) ,MDA level and SOD level .Results Compared with control group ,the APTT of Xuebijing group in 1 h and 2 h and Danshen group in 4 h after reperfusion improved significantly (P<0 .05) . The PT of Xuebijing group after reperfusion extended significantly compared with the PT before reperfusion (P<0 .05) .The FIB level of Xuebijing group and Danshen group in 4 h after reperfusion was much higher than before (P<0 .05) .The LDH and CK level of Xuebijing group after reperfusion was much lower than that of control group (P< 0 .05) .The MDA level of Xuebijing group in 2 h and Danshen group in 1 h ,2 h after reperfusion was much lower than that of control group (P<0 .05) .The SOD activ-ity of Xuebijing group in 1-4 h and Danshen group in 2 h after reperfusion was much higher than that of control group (P<0 .05) .Conclusion Xuebijing injection and Danshen injection have the relieving effect on the limb ischemic-reperfusion injury by ad-justing clotting function and decreasing free oxygen radicals .In terms of relieving the injury of muscle tissue ,the effect of Xuebijing injection might be better than Danshen .