贵州3个地域苗族人群mtDNA 9 bp序列缺失频率

摘要

目的分析贵州3个地域苗族人群线粒体DNA COⅡ/t RNALys基因间小非编码V区串联重复序列9 bp缺失频率。方法采用聚合酶链反应-聚丙烯酰胺凝胶法（PCR-PAGE）及DNA序列分析法进行检测。结果从江岜沙、黔西沙井、威宁龙街等3个地域苗族人群缺失频率分别为19．15％（9/47）、42．5％（17/40）和29．55％（13/43）；岜沙苗族人群与沙井苗族人群9 bp缺失频率比较差异有统计学意义（P＜0．05）。结论3个地域苗族人群mtDNA 9 bp缺失基因频率较高，不同地域苗族人群间缺失频率有一定差异。%Objective To analysis the frequency of 9 bp deletion polymorphism of mitochondrial DNA(mt DNA)among Miao population in three geographical regions of Guizhou.Methods Polymerase chain reaction and polyacrylamide gel electrophoresis (PCR-PAGE)were used in the study of mtDNA 9 bp deletion polymorphism,DNA sequence was used to verify the results.Results Each group who come from Basa,Shajin,Longjie in Guizhou,the frequency of 9 bp deletion of mtDNA value as 19.15%(9/47), 42.5%(17/40)and 29.55%(13/43).Short polymorphic frequency difference was statistically significant between Basa and Shajin groups(P<0.05).Conclusion The frequencies of 9 bp deletion of mtDNA are relatively high in Miao ethnic group in Guizhou. There are certain differences of the delegation frequencies among these groups.