Effects of subculture times and explant physiological characteristics on callus formation and plant regeneration in Minghui 63

摘要

The plant regeneration from indica is generallypoor.To improve the effect of the micropro- jectile and Agrobacterium-based transfor-mation of rice,subculture times and explantphysiological characteristics,which influencedthe callus formation and plant regeneration onMinghui 63,an indica variety were investigat- ed. Immature seeds of main and tillering pani-cles were collected at the 12th day after polli-