氧化苦参碱对坐骨神经结扎小鼠的镇痛作用及其对 CaMK Ⅱ受体表达的影响

摘要

Aim To observe the analgesic effect of oxymatrine(OMT)and its mechanism.Methods A peripheral mononeuropathy was produced in adult mice by placing loosely constrictive ligatures around the common sciatic nerve.The antinociceptive effects of the OMT were assessed in mechanical allodynia and cold allodynia tests.The CAMKII inhibitor KN-93 and AIP were adopted to investigate the influence of OMT on the analgesic effect and analyze its analgesic mecha-nisms.Western blot was used to evaluate the expres-sions of tCaMKII and pCaMKII protein.Results The intraperitoneal administration of OMT (1 60,80 mg· kg -1 )increased the paw withdrawal threshold in the mechanical allodynia test (P <0.05 ),OMT (1 60, 80,40 mg·kg -1 ,ip)remarkably decreased the paw lifts in the cold allodynia test (P <0.05).Ith KN-93 (1 .25 μg/site),AIP (0.02 μg/site)significantly en-hanced the analgesic effect of OMT (35 mg·kg -1 ) (P <0.01 ).Protein expression of pCaMKII was de-creased by OMT(1 60 mg·kg -1 ).Conclusion OMT has significant protective effects on chronic constriction injury(CCI)in mice,and the effective mechanism of OMT inhibits the expression of CaMKII receptor.%目的：研究氧化苦参碱（OMT）对神经病理性疼痛小鼠的镇痛作用及其机制。方法采用坐骨神经结扎模型（CCI）机械缩足反射法观察 OMT 的镇痛作用，并分析其镇痛作用与 CaMKII 受体的关系。结果①给小鼠腹腔注射160、80 mg·kg －1 OMT 可明显延长小鼠的机械缩足反射期（P ＜0.05）。②给小鼠腹腔注射160、80、40 mg·kg －1 OMT可明显降低小鼠的冷缩足反射次数（P ＜0.05）。③ OMT 能够跟 CaMK Ⅱ拮抗剂 KN-93和 AIP 发挥协同作用。④模型组小鼠的 pCaMK Ⅱ蛋白表达较假手术组明显升高（P ＜0.01）；与模型组比较，OMT（160 mg·kg －1）组可明显降低pCaMK Ⅱ蛋白的表达（P ＜0.01）。结论OMT 对神经病理性疼痛具有镇痛作用，其镇痛作用可能与 CaMK Ⅱ有关。