目的 探讨母体高盐饮食对子代肾脏血管发育的影响及其相应机制.方法 自然怀孕的Sprague-Dawley大鼠随机分为高盐组和对照组,高盐组孕鼠怀孕期间给予含8%NaCl的高盐饲料,对照组给予含1% NaCl的正常饲料,两组孕鼠均正常饮水.子代出生后,所有母鼠恢复正常饲料,新生鼠正常哺乳至1个月分笼.取成年子代公鼠肾脏叶间动脉进行血管功能检测、单个血管平滑肌(vascular smooth muscle cell, VSMC)细胞电生理实验.结果 高盐组子代肾脏小动脉对去氧肾上腺素(phenylephrine,Phe)引起的收缩反应强于对照组(P0.05).结论 孕期高盐饮食可能通过改变Phe介导的成年♂性子代肾脏小动脉血管收缩的敏感性、PKC对BK通道的调控作用,从而影响其肾脏血管功能的发育,并可能由此增加其子代肾脏疾病发生的易感性.%Aim To study the effect of high salt diet during pregnancy on the development of renal vessels in offspring rats and its mechanism.Methods Natural pregnant Sprague-Dawley rats were randomly divided into high-salt group and control group.The pregnant rats in the high-salt group were given high-salt diet of 8% NaCl content , while the control group normal diet with 1% NaCl content.In both groups, pregnant rats were given normal drinking water.After delivery, all mothers returned to normal diet and all neonatal rats were breast-fed until one month old.The adult male off springs were used as experimental animals.The vessel tone of renal interlobar arteries and electrophysiological behavior of single vascular smooth muscle cells (VSMCs) were detected respectively.Results The contractile response of renal arteries to phenylephrine(Phe) in high-salt group was stronger than that in the control group(P0.05).Conclusions High-salt diet during pregnancy could increase the sensitivity of renal interlobar arterial contractile response to Phe in adult male offsprings, which is associated with PKC-mediated BK channels pathway.Maternal high-salt diet during pregnancy may increase the risk of renal vascular diseases in adult offsprings.
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