Aim To investigate the effect of petroleum ether extract of Eclipta prostrata(PEEEP) on biochem-ical parameters such as blood glucose, insulin, total glycerides,total cholesterol,creatinine,urea nitrogen, uric acid levels and histopathology of kidney in STZ in-duced diabetic rats. Methods The model of type 2 diabetic rats was established by feeding with high ener-gy diet for 8 weeks and injecting streptozotocin(30 mg ·kg-1), after making the model successfully, the rats were randomly divided into model control group, metformin positive control group and experimental group, and gavaged with distilled water, metformin (400 mg·kg-1) and different dose of PEEEP (100, 200,400 mg·kg-1) for 4 weeks, respectively. The contents of blood glucose,insulin,total glycerides,to-tal cholesterol, creatinine, urea nitrogen, uric acid were detected by corresponding kit, the pathological changes of kidney were observed by light microscopy, and the expression of TNF-α protein was detected by Western blot. Results Compared with model control group, PEEEP could reduce blood glucose, total glyc-erides, total cholesterol, creatinine, urea nitrogen, u-ric acid and increase the content of insulin in a dose-dependent manner (P <0.01 or P <0.05). It also could alleviate the pathological damage of kidney tis-sues and down-regulate the expression of TNF-α in kidney tissues. Conclusions PEEEP could effectively improve related biochemical parameters in streptozoto-cin induced diabetic rats, and it has protective effect on the kidney, providing the theoretical basis for fur-ther exploitation and utilization of Eclipta prostrata.%目的 探究墨旱莲石油醚提取物对链脲佐菌素(strep-tozotocin,STZ)诱导的糖尿病大鼠血清中血糖、胰岛素、甘油三酯、胆固醇、肌酐、尿素、尿酸等生化指标以及肾组织病理学的影响.方法 采用高能饲料喂养8周加腹腔注射STZ (30 mg·kg-1)的方法建立2型糖尿病大鼠模型.造模成功后,随机分为模型对照组、二甲双胍阳性对照组、实验组,分别连续经口灌胃给予蒸馏水、二甲双胍(400 mg·kg-1)、墨旱莲石油醚提取物不同剂量(100、200、400 mg·kg-1)4周.利用相应试剂盒检测大鼠血清中血糖、胰岛素、甘油三酯、胆固醇、肌酐、尿素、尿酸的含量;利用光镜观察肾脏组织病理学变化;采用Western blot检测肾脏组织中TNF-α蛋白的表达.结果 与模型组相比,墨旱莲石油醚提取物各剂量均能降低血糖、甘油三酯、胆固醇、肌酐、尿素、尿酸含量,升高胰岛素含量(P<0.01或P<0.05),且呈现剂量依赖关系,同时有效减轻肾脏组织的病理损伤,下调肾脏TNF-α蛋白的表达.结论 墨旱莲石油醚提取物能有效改善STZ诱导糖尿病大鼠的相关生化指标,并对肾脏具有一定的保护作用,该研究为墨旱莲的进一步开发利用提供了理论依据.
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