＜正＞ Background Current prosthetic,small diameter vascular grafts showing poor long term patency rates have led to thepursuit of other biological materials.Biomaterials that successfully integrate into surrounding tissue should match notonly the mechanical properties of tissues,but also topography.Polyglycolic acid（70/30）has been used as syntheticgrafts to determine whether human vascular smooth muscle cells and endothelial cells attach,survive and secreteendothelin and 6-keto-prostaglandin F1α（6-keto-PGF1α）.Methods Endothelial cells and smooth muscle cells were isolated from adult human great saphenous vein.They wereseeded on polyglycolic acid scaffold in vitro separately to grow vascular patch（Groups A and B respectively）andcocultured in vitro to grow into vascular patch（Group C）.Smooth muscle cells and endothelial cells were identified byimmunohistochemical analysis and growth of cells on polyglycolic acid was investigated using scanning electronmicroscopy.The levels of endothelin and 6-keto-PGF1α in the culturing solutions were examined by radioimmunology tomeasure endothelial function.Results Seed smooth muscle cells adhered to polyglycolic acid scaffold and over 28 days grew in the interstices toform a uniform cell distribution throughout the scaffold.Then seed endothelial cells formed a complete endothelial layeron the smooth muscle cells.The levels of endothelin and 6-keto-prostaglandin F1 alpha in the culturing solution were（234±29）pg/ml and（428±98）pg/ml respectively in Group C and（196±30）pg/ml and（346±120）pg/ml in Group B;bothsignificantly higher than in Groups A and D（blank control group,all P＜0.05）.Conclusions Cells could be grown successfully on polyglycolic acid and retain functions of secretion.Our next step isto use human saphenous vein smooth muscle cells and endothelial cells to grow tubular vascular grafts in vitro.
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