Effect of CXCR4 gene overexpression mediated by lentiviral vector on the biological characteristics of mesenchymal stem cells

摘要

Objective To construct mouse CXC chemokine receptor type 4 (Cxcr4) gene overexpressing lentiviral vector and to evaluate its biological effect on mouse mesenchymal stem cells (MSCs) .Methods Cxcr4 gene was amplified and subcloned into pCR-Blunt vector.Cxcr4gene and enhanced green fluorescent protein (EGFP)