Impact of TLR4-siRNA transfection on the expression of TLR4 /MyD88 Signaling in rat tubular epithelial cells under high glucose condition

摘要

Objective To observe the expression of toll like receptor 4(TLR4)Signaling and the release of inflammation factors in rat tubular epithelial cell(NRK-52E)under high glucose condition after TLR4-siRNA transfection.Methods Three TLR4-siRNA sequences were designed and synthesized.The transfection efficiency was observed by fluorescence microscope