细粒棘球绦虫(中国大陆株)谷胱甘肽s-转移酶重组蛋白(rEgGST)的免疫保护性研究

摘要

The aim of the present study was to investigate the protective immunity against Echinococcus granulosus and its potential as vaccine candidate by using rEgGST immunizes in mice.ICR mice were randomly divided into the immunized group and the control group, and subcutaneously immunized three times.Mice were infected with protoscolices 4 weeks after immunization and sacrificed 20 weeks after infection to calculate immune protection and examine the levels in serum.Results showed that compared with the adjuvant control group, protein immunized mice's protective immunity was 89.39％.The levels of IgG, IgG's subtypes and IgE increased in varying degrees(P＜0.05).It's suggested that rEgGST could induce some protective immunity, indicating that rEgGST is a promising candidate vaccine against echinococcosis.%目的 应用细粒棘球蚴(Echinococcus granulosus,Eg)诊断抗原GST重组蛋白进行动物免疫,探讨GST免疫保护性及其作为候选疫苗的潜在价值.方法 ICR小鼠随机分为蛋白免疫组和对照组,每隔2周皮下免疫1次,在第3次免疫后4周,用Eg原头蚴进行攻击感染,感染后20周剖杀小鼠,检获棘球蚴包囊,计算免疫保护力,并用ELISA法测定血清中IgG及其亚型和IgE水平.结果 与佐剂对照组比较,蛋白免疫组小鼠的免疫保护力为89.39%;与免疫前比较,免疫后和攻击感染后蛋白免疫组小鼠血清IgG及其亚型和IgE均有不同成程度的升高(P<0.05).结论 rEgGST能诱导小鼠产生部分免疫保护力,表明rEgGST是具有发展前途的抗包虫病候选疫苗.