Objective To investigate the expression status of immune related cytokines IFN-γ, IL-10 and TGF-βmRNA in breast cancerous microenvironment and make comparison with clinicopathologic features to explore its significance.Methods Forty-eight samples of breast cancer with excised axillary lymph nodes were included.The samples were divided into two groups according to lymph node metastatic status.Digoxin-labeled oligonucleotide probes and in situ hybridization technique were used to detect the expressions of IFN-γ, IL-10 and TGF-βmRNA in breast cancer cells and the tumor infiltrating mononuclear cells.Results The expressions of TGF-βand IL-10 mRNA in breast cancerous cells were significantly higher in node metastatic group compared to those of non-metastatic group ( TGF-β[%/accumulative index]:46.25 ±12.88/0.49 ±0.27 vs 19.33 ±7.69/0.28 ±0.18; IL-10[%/accumulative index]: 39.08 ±17.49/0.42 ±0.19 vs 22.58 ±7.87/0.33 ±0.17, t=4.330, P<0.01;t=2.962, P<0.05).The difference in expression of TGF-βmRNA in breast cancer cells was more significant.IFN-γmRNA positive cancer cell was not detected.In tumor infiltrating mononuclear cells, the IFN-γmRNA positive cells in node metastatic group were significantly less than TGF-βand IL-10 mRNA positive cell (TGF-β:35.58 ± 13.62/0.40 ±0.25, IL-10:40.83 ±15.49/0.47 ±0.22, IFN-γ:18.17 ±8.41/0.18 ±0.08, t=3.005, P<0.05;t=4.390, P<0.01;t=3.563, P<0.01).Conclusion Treg and Th2 related cytokines are predominated in local microenvironment of breast cancer and the microenvironments of breast cancer demonstrate an immunoinhibitory status in terms of anticancer immune response.%目的 研究乳腺癌局部微环境中免疫相关细胞因子干扰素(IFN)-γ、白介素(IL)-10和转化生长因子(TGF)-βmRNA表达的状况,并与临床病理特征相比较,探讨其意义.方法 48例乳腺浸润性导管癌手术切除标本,根据是否淋巴结转移分为两组,采用地高辛末端标记的寡核苷酸探针,以原位杂交技术检测肿瘤浸润单个核细胞和肿瘤细胞IFN-γ、IL-10和TGF-βmRNA的表达状况.结果 淋巴结转移组乳腺癌细胞TGF-β和IL-10 mRNA表达明显高于无淋巴结转移组(TGF-β[%/积分指数]:46.25±12.88/0.49±0.27 vs 19.33±7.69/0.28±0.18;IL-10[%/积分指数]:39.08±17.49/0.42±0.19 vs 22.58±7.87/0.33±0.17,t=4.330,P<0.01;t=2.962,P<0.05),TGF-βmRNA在乳腺癌细胞中表达的差别更为明显.未检出IFN-γmRNA阳性肿瘤细胞.肿瘤间质浸润的单个核细胞中,淋巴结转移组IFN-γmRNA阳性细胞明显低于TGF-β、IL-10 mRNA阳性细胞(TGF-β:35.58±13.62/0.40±0.25,IL-10:40.83±15.49/0.47±0.22,IFN-γ:18.17±8.41/0.18±0.08,t=3.005,P<0.05;t=4.390,P<0.01;t=3.563,P<0.01).结论 乳腺癌组织局部微环境中,调节T细胞和Th2免疫相关细胞因子mRNA表达明显占优势,使得肿瘤免疫微环境趋于抗瘤免疫反应抑制状态.
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