拟南芥AtLURP1基因启动子在转基因烟草和水稻中的功能分析

摘要

克隆拟南芥LURP1基因上游1515 bp的启动子调控序列并命名为AtLURP1p,将其与GUS报告基因融合,构建成植物表达载体,分别遗传转化烟草和水稻,获得LURP1p::GUS的烟草和水稻转基因植株及其相应的T2代株系,分别研究LURP1p对水稻稻瘟病、辣椒青枯病侵染及SA、MeJA、ABA等几种重要的植物激素信号分子处理的应答.结果表明:(1)转基因烟草和水稻在几种激素,包括SA、MeJA、ABA的诱导处理下,GUS基因均可以被诱导表达；(2)转基因烟草在细菌性病菌青枯病的侵染下,GUS可被诱导表达并表现出持续表达的趋势,转基因水稻在稻瘟病的侵染下,其GUS基因也被诱导表达,并表现出后期持续上调的趋势.这些研究结果表明,拟南芥LURP1的应答逆境信号通路也存在于烟草和水稻等植物,该启动子可用作诱导型启动子广泛地应用于不同植物抗病基因工程.%The 1 515 bp upstream promoter regulating sequence of Arabidopsis thaliana LURP1 (Late Unregulated in Response to HyaLoperonospora parasitka) gene was cloned and named as AtLURPlp. The promoter was fused to the GUS reporter gene to generate a expression vector and to be transformed to tobacco and rice. The T2 transgenic plants were used for expression character analyses of LURP1 promoter in response to rice blast, pepper Ralslonia solanacearum and many important plant hormone signal molecules, including SA, MeJA and ABA. The results revealed that the GUS gene driven by LURP1 promoter could be induced by exogenous hormone treatment, including SA, MeJA and ABA in transgenic tobacco and rice; LURP1-driven GUS gene could be induced and exhibited late up-regulated in response to Ralstonia solanacearum infection in transgenic tobacco; Similarly in transgenic rice the GUS driven by LURP1 promoter could also be induced and late up-regulated. These findings indicated that the signaling pathway of LURP1 in Arabidopsis thaliana also consists in tobacco and rice. The promoter of LURP1, as an inducible promoter could be widely applied in disease-resistant gene engineering of different plants.