利用代谢组学方法分析生防菌抑菌机理模式的初步建立

摘要

In this investigation,the antifungal modes of action of nine fungicide with different targets or modesof-action on Fusarium oxysporum were studied.Metabolic profiles of cultures treated with nine different fungicide were acquired by LC/Q-TOF MS,significant metabolic markers were used for classification by PCA analysis,and the fungicide could be divided into six groups.Using the six groups as training sample set,PLS-DA prediction model were established.Confirmed with the model,the conclusion could be drawn that the antifungal mode of action of Brevibacillus brevis,a biocontrol strain of Cucumber Fusarium Wilt,is similar to that of azoxystrobin and Cyazofamid,which act as respiratory inhibitor.%应用9种杀菌剂来处理尖孢镰刀菌,通过HPLC/Q-TOF方法进行代谢轮廓分析.结果表明:不同菌剂处理后的尖孢镰刀菌代谢组显著差异,PCA分析可将其分为6组.将6组杀菌剂作为训练样本集,建立杀菌剂对尖孢镰刀菌抑菌机理的PLS-DA模型.以枯萎病生防菌短短芽胞杆菌为测试样本,利用该模型对其活性物质进行了抑菌机理的分析判别,发现其作用方式与嘧菌酯、氰霜唑类似,即通过抑制病原菌的呼吸作用而发挥抑菌功能,说明利用该模型判别生防菌对尖孢镰刀菌的抑菌机理是可行的.