mPEG-BTC体外修饰红细胞抗原解决疑难交叉配血的初步研究分析

摘要

Objective:To explore the methoxy polyethylene glycol -benzotriazole ( mPEG-BTC) erythrocyte antigens in vitro modifi-cation troubleshoot crossmatch role .Methods:The modified with mPEG -BTC 12 healthy blood donors and 14 cases of red blood cells cross matching blood samples erythrocyte difficult to observe mPEG -BTC for healthy donors erythrocyte blood group antigen masking effect, compared mPEG-BTC healthy blood donors before and after modification red blood cells and difficult cross matching blood sam -ples with blood results and comparison with clinical results with blood .Results:mPEG -BTC fully shielded healthy donors erythrocyte blood group antigens , modified erythrocytes and difficult cross matching blood samples did not appear agglutination , difficult cross-matc-hing of blood samples modified with mPEG -BTC red blood cross matching consistency was higher than clinical cases with blood (P <0. 05).Conclusion:mPEG-BTC vitro modified red blood cell antigens could effectively solve the difficult problem of cross -matching of blood, worthy of promotion .%目的：探究甲氧基聚乙二醇-苯丙三唑（ mPEG-BTC）体外修饰红细胞抗原解决疑难交叉配血的作用。方法：分别用mPEG-BTC修饰12例健康献血者的红细胞和14例疑难交叉配血标本红细胞，观察mPEG-BTC对健康献血者的红细胞血型抗原的遮蔽作用，比较mPEG-BTC修饰前后健康献血者红细胞与疑难交叉配血标本的配血结果，并与临床配血结果比较。结果：mPEG-BTC完全遮蔽健康献血者的红细胞的血型抗原，修饰后的红细胞与疑难交叉配血标本未出现凝集反应，疑难交叉配血标本与mPEG-BTC修饰后红细胞交叉配血相合率高于临床配血情况（ P＜0．05）。结论：mPEG-BTC体外修饰红细胞抗原能有效解决疑难交叉配血的问题，值得推广。