Objective:To explore the protective effect of Jiaotai Pill on type 2 diabetes mellítus (T2DM) of db/db mice kidney and the regulation effect on PPAR gene expression in of adipose tissue.Methods:db/db mice were divided into model group,Jiaotai Pill group and rosiglitazone group,and db/m mice were set as normal control group,each contain 8 mice.Each group was given the corresponding medicine or saline for 4 weeks,food and water intake,urine volume,body weight,fasting blood glucose (FBG),blood lipid and urine indexes were detected,the kidneys were stained with HE to observe the pathological changes and the expression of PPARγmRNA in adipose tissue was detected by RT-PC1.Results:Jiaotai Pill significantly improved the food intake,water intake,urine volume,FBG,triglyceride (TG) and urine indexes of db/db mice (P ＜ 0.05);up -regulated the expression on PPARγin adipose tissue(P ＜ 0.05);protected renal tissue from injury.Conclusion:Jiaotai Pill not only can effectively improve the symptoms of diabetes in db/db mice,but also can inhibit the weight gain and increase PPARγmRNA expression,repair the kidney damage in db/db mice.%目的:观察交泰丸对2型糖尿病(T2DM)db/db小鼠肾脏的保护作用以及对脂肪组织中PPARγ基因表达的影响.方法:db/db小鼠分为模型组、交泰丸组和罗格列酮组,另取db/m小鼠为正常对照组;每组8只.给药处理4周,观察小鼠的饮食摄入量、尿量、体质量、空腹血糖(FBG)、血清三酰甘油(TG)、总胆固醇(TC)以及尿液指标变化,取肾脏进行HE染色观察其病理改变,RT-PCR检测脂肪组织PPARγmRNA表达.结果:交泰丸能够显著改善db/db小鼠饮食摄入量、尿量、FBG、血清三酰甘油(TG)以及尿液指标(P＜0.05);上调脂肪组织中PPARγ的基因表达水平(P＜0.05);减轻db/db小鼠的肾组织损伤.结论:交泰丸可有效地改善db/db小鼠的糖尿病症状,而且可以在不增加体质量的同时上调PPARγ的基因表达,修复db/db小鼠的肾损伤.
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