大节段生物活性陶瓷多孔支架肌内血管化:3年期3D-数字减影血管造影观察

摘要

BACKGROUND: The core of the artificial bone material activating, biological bonding with autologous bone and completion of autologous bone creeping substitution in vivo is vascularization. The occurrence of vascularization is still unclear. OBJECTIVE: To observe the vascularization of the large segment of bioactive ceramic scaffold implanted into the skeletal muscle for 3 years.METHODS: Ten male dogs were selected for implanting porous hydroxyapatite-polylactic acid scaffold (diameter of 1.5 cm, length of 4-4.5 cm) in skeletal muscles. Thirty-six months later, three-dimensional digital subtraction angiography (3D-DSA) was performed.RESULTS AND CONCLUSION: All the dogs underwent operation and 3D-DSA successful and the results were similar. 3D-DSA showed: nutrient arteries derived from the femoral artery and deep femoral artery and pointed to the hydroxyapatite-polylactic acid scaffold, the branches inserted into the hydroxyapatite-polylactic acid scaffold. 3D-DSA is an effective means of observation of vascularization of artificial bone in vivo. The integral arterial and venous blood circulatory system can be established in hydroxyapatite-polylactic acid scaffold implanted in skeletal muscle for 3 years.%背景 人工骨材料生物体内的活化,与自体骨生物键合,完成自体骨组织爬行替代,核心是材料的血管化.如何判断血管化发生?目的 观察大节段生物活性陶瓷多孔支架骨骼肌内植入3 年后血管化情况.方法 雄性中国自养犬10 只,于骨骼肌内植入直径1.5 cm、长4.0~4.5 cm 羟基磷灰石-聚乳酸多孔支架,饲养36 个月后行3D 数字减影血管造影检查.结果 与结论 所有动物均顺利完成材料植入及数字减影血管造影成像,且所得结果相似:营养血管由股动脉、股深动脉发出并指向羟基磷灰石-聚乳酸支架,分支后进入材料.结果 表明,3D 数字减影血管造影技术是用于监测人工骨材料体内血管化的有效手段,羟基磷灰石-聚乳酸支架骨骼肌内植入3 年后能够形成完整的动静脉血循环系统.