复方丹参对高脂大鼠模型骨形态计量学参数的影响**

摘要

背景：复方丹参对抗泼尼松性大鼠的骨丢失具有抑制骨吸收作用，但对高脂血症所致的骨质疏松症效果如何未见报道。目的：通过骨形态计量学观察复方丹参对大鼠胫骨和腰椎骨丢失的防治作用。方法：用脂肪乳剂建立高脂血症骨丢失大鼠模型，30只SD大鼠随机数字表法分为3组，正常对照组、高脂乳剂组、复方丹参组，分别给予生理盐水、高脂乳剂、高脂乳剂和复方丹参，连续16周。结果与结论：与高脂乳剂组相比，复方丹参可使高脂大鼠胆固醇明显降低和高密度脂蛋白胆固醇明显升高，可以有效改善高脂乳剂导致的脂质代谢紊乱。复方丹参组的骨小梁面积百分数、骨小梁数量、成骨细胞贴壁长度百分率均增加(P <0.05)，而骨小梁分离度、每毫米破骨细胞数、破骨细胞贴壁长度百分率均减少(P <0.01)，反映骨形成及骨矿化的指标变化不明显；胫骨中段的皮质骨面积百分数增加(P <0.01)，骨髓腔面积百分数减少(P<0.01)，骨内外膜的形成和矿化均无明显变化；腰椎松质骨的骨小梁面积百分数、骨小梁数量、骨小梁宽度增加(P<0.05)、而骨小梁分离度减少(P<0.01)。提示长期高脂乳剂灌胃会导致大鼠骨量丢失，复方丹参能有效对抗高脂大鼠胫骨上段、中段骨、第5腰椎的骨丢失。%BACKGROUND:Compound danshen extract can inhibit bone resorption and rival bone loss of rats induced by prednisone. However, there are no reports indicating its effectiveness in osteoporosis induced by hyperlipidemia. OBJECTIVE:To study the effect of compound danshen extract against tibia and lumbar spine bone loss by using bone histomorphometry through establishing a osteoporosis rat model induced by high-fat emulsion. METHODS:Thirty 3-month-old male Sprague-Dawley rats were randomly divided into normal saline group, high-fat emulsion group, compound danshen extract group. Rats were sacrificed after 16 weeks. RESULTS AND CONCLUSION:Compared with the high-fat emulsion group, serum total cholesterol decreased and high-density lipoprotein cholesterol increased significantly fol owing treatment with compound danshen extract. In the longitudinal proximal tibial metaphyseal of rats treated with compound danshen extract,%Tb.Ar, Tb.N and%Ob.S.Pm increased (P<0.05), while Tb.Sp,%Oc.S.Pm and Oc.N/mm decreased (P<0.01). In the tibial shaft,%Ct.Ar increased (P<0.01), while%Ma.Ar decreased (P<0.01). In the fifth lumbar vertebral body,%Tb.Ar, Tb.N and Tb.Th increased (P<0.05), while Tb.Sp decreased (P<0.01). Long-term high-fat diets can cause the loss of bone in rats, and compound danshen extract can prevent these changes through stimulating periosteal bone formation.