构建腰椎间盘突出症血瘀证的血清蛋白指纹图谱模型

摘要

背景：腰椎间盘突出症血瘀证与非血瘀证的相互关系尚不明确。　　目的：构建腰椎间盘突出症血瘀证的血清蛋白指纹图谱模型。　　方法：按照病例对照研究方法，遵循组间民族、性别及年龄等相匹配原则筛选180例研究对象，其中120例分为腰椎间盘突出症血瘀证组及腰椎间盘突出症非血瘀证组，每组60例；健康对照组60例。抽取受试者外周血的血清样本，应用表面增强激光解吸/电离飞行时间质谱及蛋白质芯片技术检测并绘制蛋白质质谱图，随后用Biomarker Wizard软件识别蛋白峰信息，建立腰椎间盘突出症血瘀证的血清诊断模型，并通过双盲验证法对模型进行验证及通过ExPASy数据库对相关差异蛋白进行蛋白检索。　　结果与结论：在腰椎间盘突出症血瘀证、腰椎间盘突出症非血瘀证及健康者各组之间找到11个蛋白质峰有统计学意义(P<0.05)，其中2个蛋白质呈高表达、9个蛋白质呈低表达；用Biomarker Patterns Software构建腰椎间盘突出症血瘀证血清学诊断模型并验证，其敏感性为86.667%，特异性为94.167%，阳性预测值为88.136%。表明腰椎间盘突出症血瘀证患者的血清中存在多种异常表达的蛋白质；由11个差异蛋白组成的血清蛋白质指纹图谱模型可作为腰椎间盘突出症血瘀证的血清标志物诊断模型。%BACKGROUND:The correlation between blood stasis syndrome and non-blood stasis syndrome of lumbar intervertebral disc protrusion remains unclear. OBJECTIVE:To construct serum protein pattern model for diagnosing blood stasis syndrome of lumbar intervertebral disc protrusion. METHODS:A total of 180 cases were included in this study and divided into treatment group (120 patients with lumbar intervertebral disc protrusion) and control group (60 healthy cases from physical examination). Furthermore treatment group was equal y assigned into blood stasis syndrome subgroup and non-blood stasis syndrome subgroup, with 60 cases in each subgroup. The involved cases were wel matched in nations, genders and ages. Serum samples of peripheral blood from the 180 cases were col ected. Surface-enhanced laser desorption/inionation time of flight mass spectrometry and ProteinChip technology were employed to detect and plot protein mass spectrum. The protein peak values were identified using Biomarker Wizard software. Then serum diagnosis model of blood stasis syndrome of lumbar intervertebral disc protrusion was established. The obtained models were verified through double blind method. The differential proteins were searched by ExPASy data. RESULTS AND CONCLUSION:We detected that peak values of eleven proteins had statistical significance (P<0.05) from the involved 180 cases. Among them, two proteins were highly expressed while the other nine proteins were lowly expressed. Serum protein pattern model for diagnosing blood stasis syndrome of lumbar intervertebral disc protrusion was established through Biomarker Patterns software, and the sensibility was 86.667%, the specificity was 94.167%, the positive predictive value was 88.136%. There are a variety of abnormal y expressed proteins in the serum of the patients with blood stasis syndrome of lumbar intervertebral disc protrusion. The serum protein pattern model involved eleven different proteins can be used to diagnose blood stasis syndrome of lumbar intervertebral disc protrusion.