水产养殖环境中病原菌是导致水产养殖病害的主要原因,针对传统方法存在耗时长、试剂成本高、目标菌难分离及自动化程度低等缺点,开展了面向水产病原菌微流控检测的磁控分离方法的研究.设计了磁控捕获及分离等功能的专用磁场控制器,构建了基于微流控芯片的磁控分离实验平台,优化选择了磁极电流、磁控频率及捕获时间等参数,并以大肠杆菌E.coli O157:H7为例对磁控分离捕获率进行了实验验证,研究结果表明,磁控分离法捕获率可达到92%以上,与被动分离捕获方式相比,不仅捕获率提高30%,而且分离操作灵活可控,自动化水平较高,实现了水产病原菌的高效分离,也为后续水产养殖病害快速检测与预警提供保障.%Pathogenic bacteria in aquatic environment is the major cause of aquaculture diseases.However conventional methods require a long time and a great amount of cost for other reagents,and suffer from difficult isolation and lower automation.A magnetic isolating method for aquaculture pathogens detection system was proposed.A dedicated magnetic controller with magnetic capture and isolation was designed,and the magnetic isolation experiment platform based on microfluidic chip was built up.The optimum magnetic pole current,switching frequency and capture time of the platform were determined,and illustrated by the case of Escherichia coli O157:H7 the performance of the method was experimentally verified.The research results showed that the capture efficiency of magnetic isolating method reached as high as 92%.And compared with passive isolation by barriers,the capture efficiency increased by 30%,and the separating operation was flexible and controllable with higher automation,which realized pathogens efficient separation and benefitted rapid detection and early warning on aquaculture disease.
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