基于微环形阵列电极的细胞分离富集芯片的设计与实验研究

摘要

根据介电泳操作原理,设计了微环形阵列电极结构,建立了细胞分离富集芯片模型,采用COMSOL软件分析微环形阵列电极的电场分布和介电泳力方向并确定了最大和最小电场强度的位置,利用ITO玻璃和PDMS制备了细胞分离富集芯片.通过酵母菌细胞的介电泳富集实验和酵母菌细胞与聚苯乙烯小球的分离富集实验,明确了酵母菌细胞的临界频率,实现了酵母菌细胞和聚苯乙烯小球的分离富集.结果显示,在溶液电导率为60μs/cm,交流信号电压为8Vp-p时,酵母菌细胞在1kHz~45kHz频率范围内做负介电泳运动并富集在环形内部,45kHz为酵母菌细胞的临界频率,在45kHz~10MHz频率范围内做正介电泳运动并富集在环形边缘;1.5MHz时聚苯乙烯小球做负介电泳运动并富集在环形内部,富集倍数达到11.66.%According to the theory of dielectrophoresis,a kind of cells isolation and enrichment chip of micro annular array was designed and modeled.COMSOL software was used to discover the position of maximum and minimum strength of electric field via analysing the distribution of electric field and the direction of dielectrophoresis force.The chip was fabricated based on ITO glass and PDMS.The cross frequency of yeast cells was obtained after conducting a dielectrophoretic enrichment experiment of yeast cells.Isolation and enrichment of yeast cells and polystyrene particles were realized through carrying out an isolation and enrichment experiment with yeast cells and polystyrene particles.The results showed that yeast cells in solution conductivity of 60 μs/cm did negative DEP response and enriched in the ring inside under a voltage of 8 V p-p and the frequency of 1 kHz to 45 kHz.The cross frequency of yeast cells was determined to be 45 kHz.Yeast cells did positive DEP response and enriched along the ring edge in the range of 45 kHz to 10 MHz.The polystyrene particles did negative DEP response and enriched in the ring inside under a frequency of 1.5 MHz.The multiple of enrichment reached about 11.66.