超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响

摘要

Objective To explore the effect of ultra-early hyperbaric oxygenation (HBO) on bone calcium, biomechanical properties and bone collagen of femur in rats with complete spinal cord transaction. Methods A total of 75 Sprague-Dawley rats were randomly divided in-to sham group (n=15), model group (n=20) and HBO group (n=40). HBO group was divided into three hours group (HBO1 group, n=20) and twelve hours group (HBO2 group, n=20). All groups underwent laminectomy at T10, while the model group, HBO1 group and HBO2 group underwent complete spinal cord transection at the same level. Three hours and twelve hours after surgery, HBO1 group and HBO2 group received HBO, respectively, for three courses with ten days in a course. After treatment, the femoral biomechanical properties, bone calcium and hydroxyproline (Hyp) were determined. The morphology of bone trabecula and the bone collagen was observed with HE stain-ing and Masson triad color staining, respectively. Results After treatment, compared with the sham group, the femoral biomechanical proper-ties, the content of bone calcium and Hyp decreased in the model group (P<0.05);compared with the model group and HBO2 group, they in-creased in HBO1 group (P<0.05). The number of bone trabecula and the bone collagen decreased, and derangement and sparseness were ob-served in the model group;however, the changes were substantially mild in HBO1 group. Conclusion Ultra-early HBO could increase the content of bone calcium and Hyp of femur, improve the morphology of the femur bone collagen, and improve the femoral biomechanical properties in rats with complete spinal cord transection.%目的：探讨超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响。方法75只Sprague-Dawley大鼠随机分为假手术组(n=15)、模型组(n=20)和高压氧组(n=40)，其中高压氧组又分为3小时组(高压氧1组，n=20)和12小时组(高压氧2组，n=20)。假手术组仅行椎板切除术，其他三组均行T10水平脊髓完全横向切断术。高压氧1组和高压氧2组分别于术后第3、12小时开始高压氧治疗，10 d为1个疗程，共3个疗程。治疗后，各组取右股骨测定股骨生物力学性能参数，测定骨钙、骨羟脯氨酸(Hyp)含量；HE染色和Masson染色观察骨小梁和骨胶原形态学变化。结果治疗后，与假手术组比较，模型组股骨结构力学和材料力学参数下降(P<0.05)，骨干重、骨钙及Hyp含量减少(P<0.05)；骨小梁及骨胶原数目减少，稀疏、断裂、排列不整。与模型组和高压氧2组比较，高压氧1组股骨结构力学和材料力学参数均增高，骨干重、骨钙及Hyp含量增加(P<0.05)，骨小梁及骨胶原数目增多，连续性较好，排列较整齐。结论超早期高压氧治疗可明显改善脊髓全横断损伤大鼠股骨骨钙含量，改善骨胶原含量及质量，改善生物力学性能。