血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系

摘要

目的 测定急性脊髓损伤大鼠血清中二胺氧化酶(DAO)的含量,同时观察肠道运动功能的变化和肠黏膜屏障损伤的程度.方法 45只Sprague-Dawley大鼠随机分为打击组(A组)、假手术组(B组)和对照组(C组),每组15只.采取改良Allen打击法(10 g×25 mm)损伤大鼠T10脊髓节段.术后1 d、3 d、7 d分别对各组大鼠进行后肢功能BBB评分,测定大鼠回肠肌电慢波及平滑肌收缩,并做回肠HE染色,ELISA试剂盒测定血清DAO含量.结果 脊髓损伤后1 d、3 d和7 d,A组BBB评分显著低于C组(P<0.001),B组与C组无显著性差异(P>0.05).脊髓损伤后1 d、3 d,A组慢波频率和振幅低于C组(P<0.05),收缩频率和振幅低于C组(P<0.05),B组与C组无显著性差异(P>0.05);脊髓损伤后7 d,三组肌电慢波频率和振幅、收缩的频率和振幅均无显著性差异(P>0.05).A组回肠黏膜水肿、倒伏,炎性细胞浸润,黏膜下间隙增大.A组肠黏膜损伤Chiu评分高于B组和C组(P<0.05).脊髓损伤后1 d、3 d,A组大鼠血清DAO含量高于C组(P<0.05),B组与C组无显著性差异(P>0.05);脊髓损伤后7 d,三组间血清DAO含量均无显著性差异(P>0.05).结论 血清DAO含量可以反映大鼠急性脊髓损伤后肠道的运动功能和黏膜损伤程度.%Objective To measure the level of diamine oxidase (DAO), and observe the intestinal motor and mucosal barrier injury after acute spinal cord injury (SCI) in rats. Methods A total of 45 Sprague-Dawley rats were randomly divided into SCI group (group A, n=15), sham group (group B, n=15) and control group (group C, n=15). SCI model was established with Allen's strike mode (10 g × 25 mm) by striking T10 spinal segment in rats. One day, three days and seven days after SCI, hind limb motor function was assessed with Basso-Beat-tie-Bresnahan (BBB) Scale in each group, the myoelectric slow wave and ileum smooth muscle contractility were measured in rats, ileum tis-sues were tested with HE staining, and the DAO content of serum was tested with ELISA kit. Results One day, three days and seven days af-ter SCI, the BBB score was significantly lower in group A than in groups B and C (P<0.001). One day, three days after SCI, the frequency and amplitude of both slow wave and contractility were lower in group A than in groups B and C (P<0.05);seven days after SCI, there was no significant difference among three groups (P>0.05). Group A showed ileal mucosal edema, lodging, inflammatory cell infiltration, and submucosal gap increase. The Chiu's score of intestinal mucosal injury was higher in group A than in groups B and C (P<0.05), as well as the serum DAO content one day and three days after SCI (P<0.05), and no significant difference was found in the serum DAO content among three groups seven days after SCI (P>0.05). Conclusion Serum DAO content may respond to the intestinal motor function and mu-cosal injury after acute SCI in rats.