神经细胞黏附分子NB-3在调节胚胎来源神经干细胞分化和迁移中的作用

摘要

目的:探讨NB3基因在调节小鼠探讨神经干细胞分化和迁移中的作用.方法:分离培养来源于E14.5d NB3缺失和野生型小鼠海马的神经前体细胞,通过免疫荧光染色方法检测NB3缺失对神经干细胞(NSCs)诱导分化后神经元分化和迁移的影响.结果:①NB3在体外培养的神经干细胞中表达,并和干细胞的标记分子Nestin共定位；②NB3基因缺失后对神经干细胞的体外增殖能力无明显影响,但在用1％胎牛血清诱导分化后,NB3缺失的神经干细胞产生更多的神经元；③与对照组相比,从NB3基因缺失的神经干细胞分化而来的神经元表现为相互聚集而不向神经球外迁移.结论:NB3在神经元的再生和迁移中具有一定的调节作用.%Objective: To investigate the effects of NB3 on regulating differentiation and migration of embryonic neural stem cells(NSCs).rnMethod: The NSCs were isolated and cultivated respectively from E14.5d NB-3-deficient mice and wide type cells. The effects of NB3 on regulating differentiation and migration of NSCs were detected by immunofluores-cence.rnResult: NB-3 was highly expressed in NSCs derived from E14.5d hippocampus. There was no significant difference of proliferation of NSCs between NB-3 deficient mice and wild type mice; However, the NB-3 deficient NSCs differentiated into more neurons than those from wild type cells, and the neurons differentiated from NB-3 deficient NSCs clustered and leas migrated to outsidc of neurospheres compared with those from wild type cells. Conclusion: NB-3 might be a novel factor which regulated the differentiation of NSCs and the migration of neurons.