牛病毒性腹泻病毒基因Ⅱ型HLJ-10分离株全基因组克隆及其序列特征分析

摘要

The complete genome of a bovine viral diarrhea virus (BVDV) II isolate was cloned and sequenced. The genome of the isolate was 12,284 nucleotides (nt) in length, which contained a 11,694 nt open reading frame with 22 potential N-glycosylation sites, a 385 nt 5'untranslated region (UTR) and a 205 nt 3XUTR. The complete genomic sequence of HLJ-10 was submited into GenBank. Comparing to other reference strains, the isolate of HLJ-10 had a high sequence identity with reference strains of SH-28 and KZ91CP.%为研究牛病毒性腹泻病毒(BVDV)的分子特征,本研究参考GenBank中登录的BVDV基因Ⅱ型病毒株全基因组序列,设计多对引物,通过RT-PCR方法,对一株分离自某商品化血清中的BVDV分离株HLJ-10全基因组进行分段克隆及序列测定,获得了该分离株的全基因组序列.生物信息学分析表明,该分离株为BVDV基因Ⅱ型,全长12284nt,编码区为11694nt,共编码3897个氨基酸,5’和3’非编码区分别为385 nt和205 nt.与其它BVDV参考株序列比对分析表明,HLJ-10与SH-28和KZ91CP进化关系较密切,预测该分离株含有22个潜在糖基化位点,氨基酸差异性分析表明E2蛋白的氨基酸序列在瘟病毒属中变异性较大.