IL-6通过诱导lncTCF7表达促进甲状腺乳头状癌TPC-1细胞上皮-间充质转化、迁移和侵袭

摘要

目的:探讨白细胞介素6 (IL-6) 通过诱导长链非编码RNA lnc TCF7表达对人甲状腺乳头状癌TPC-1细胞上皮-间充质转化 (EMT) 、迁移和侵袭能力的影响.方法:以0、5、10、20和50μg/L的IL-6作用于TPC-1细胞24 h或以50μg/L的IL-6作用于TPC-1细胞0、6、12和24 h后, RT-qPCR检测IL-6对TPC-1细胞中lnc TCF7表达的影响.以50μg/L的IL-6处理TPC-1细胞24 h后, Western blot检测IL-6对TPC-1细胞中E-cadherin和vim-entin蛋白表达的影响.建立lnc TCF7过表达的TPC-1细胞株, Western blot和Transwell小室实验检测过表达lnc TCF7对TPC-1细胞EMT、迁移和侵袭能力的影响.敲减lnc TCF7的表达并给予50μg/L的IL-6处理后, 观察lnc TCF7对IL-6处理后的TPC-1细胞EMT、迁移和侵袭能力的影响.结果:IL-6可呈剂量和时间依赖性地诱导TPC-1细胞中lnc TCF7的表达 (P <0.05) .过表达lnc TCF7可下调TPC-1细胞中E-cadherin表达, 上调vimentin表达, 增强细胞的迁移和侵袭能力 (P <0.05) .IL-6可使TPC-1细胞中E-cadherin表达降低, vimentin、Snail和Slug表达升高, 同时, 增强细胞的迁移和侵袭能力, 增大细胞间隙;敲减lnc TCF7表达能够明显抑制IL-6引起的上述变化.结论:IL-6可通过诱导lnc TCF7表达促进甲状腺乳头状癌TPC-1细胞的EMT、迁移和侵袭.%AIM:To investigate the effect of interleukin-6 (IL-6) on epithelial-mesenchymal transition (EMT) , migration and invasion of papillary thyroid carcinoma TPC-1 cells by inducing the expression of long noncoding RNA lnc TCF7.METHODS:The effects of IL-6 on the expression of lnc TCF7 in the TPC-1 cells were detected by RT-q PCR after the TPC-1 cells were treated with IL-6 at 0, 5, 10, 20 and 50μg/L for 24 h or with IL-6 at 50μg/L for 0, 6, 12 and 24 h.After the TPC-1 cells were treated with IL-6 at 50μg/L for 24 h, the effect of IL-6 on the protein expression of E-cadherin and vimentin in the TPC-1 cells was detected by Western blot.The TPC-1 cell line with lnc TCF7 over-expression was established, and the effects of lnc TCF7 over-expression on EMT, migration and invasion of the TPC-1 cells were measured by Western blot and Transwell assay.After knockdown of lnc TCF7 expression and exposure to IL-6 at 50μg/L, the effects of lnc TCF7 on EMT, migration and invasion of TPC-1 cells treated with IL-6 were observed.RESULTS:The expression of lnc TCF7 in the TPC-1 cells was induced by IL-6 in a dose-and time-dependent manner.The expression of E-cadherin was down-regulated, the expression of vimentin was up-regulated, and the migration and invasion abilities of the TPC-1 cells were enhanced by lnc TCF7 over-expression (P<0.05).The expression of E-cadherin was decreased, the expression of vimentin, Snail and Slug was increased, and the migration and invasion abilities of the TPC-1 cells and intercellular space were enhanced by IL-6.The above changes induced by IL-6 were significantly inhibited by knockdown of lnc TCF7expression.CONCLUSION:IL-6 promotes the EMT, migration and invasion of papillary thyroid carcinoma TPC-1 cells by inducing the expression of lnc TCF7.