补肾活血泄浊汤治疗大鼠局灶性节段性rn肾小球硬化的研究

摘要

AIM: To observe the effects of Decoction of Bu-Shen-Huo-Xue-Xie-Zhuo (BSHXXZ) on focal segmental glomerulosclerosis (FSGS) in rats. METHODS: Unilateral nephrectomy was adopted firstly, one week later adrimycin was intravenously adminstered to 20 rats twice in a 23-day period to establish an advanced focal segmental glomerulosclerosis model in the rat. Changes in urine protein, blood chemistry, and histology of the kidney were investigated for 110 days after unilateral nephrectomy. RESULTS: The BSHXXZ decoction reduced edema, proteinuria, hyperlipidemia, azotemia and ascites, and increased albumin in blood. Light microscopic, electron microscopic, immunohistochemical and polarizing microscopic examination all showed that the pathologic changes in the treatment group were less than that of the model group. CONCLUSION: Decoction of BSHXXZ could markedly improve renal function and delay the progression of glomerulosclerosis and renal interstitial fibrosis.%目的：观察中药补肾活血泄浊汤对大鼠局灶性节段性肾小球硬化的治疗作用及作用机制。方法： 采用单侧肾切除术，两次尾静脉注射阿霉素法复制局灶性节段性肾小球硬化模型，分为补肾活血泄浊汤治疗组、模型组和正常组。实验的前6周每周测大鼠24 h尿蛋白，后6周隔周测大鼠24 h尿蛋白，第60 d,100 d时测定血清白蛋白、胆固醇、肌酐、尿素氮含量，并进行光镜、电镜、原位杂交、免疫组化等观察。结果：治疗组各项指标与模型组相比均有显著性差异，形态学观察也显示治疗组损害轻于模型组。 结论： 补肾活血泄浊汤能够明显保护病鼠肾功能，明显减轻病鼠细胞外基质沉积，延缓病变肾小球硬化的发生。