miRNA-22在卵巢癌组织的表达及其对卵巢癌细胞增殖、迁移与侵袭的影响

摘要

AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells.METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR .miRNA-22 was over-expressed by trans-fection of miRNA-22 mimic.The cell viability was examined by CCK-8 assay.The cell migration was measured by wound healing test .The cell invasion was analyzed by Transwell assay .The protein expression levels of VEGF and P 53 were deter-mined by Western blot .RESULTS: Compared with the normal ovarian tissue , the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues .After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased , while the cell viability , migration and invasion were obviously decreased .Moreover , the protein expression of VEGF and P 53 was dramatically inhibited after over-expression of miRNA-22.CONCLUSION:The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer.Over-expression of miRNA-22 decreases the cell viability , migration and invasion by reducing the protein expression of VEGF and P53.%目的：观察在不同卵巢组织中miRNA-22的表达，并研究上调miRNA-22表达对卵巢癌细胞株SK-OV-3增殖、迁移与侵袭的影响。方法：实时荧光定量PCR（ qPCR）检测不同卵巢组织样本miRNA-22的表达；随机将SKOV-3细胞分为正常对照组（ control组）、空白载体组（ miRNA-22-NC组）和转染miRNA-22-mimic组（ miRNA-22-m组），qPCR检测各组细胞miRNA-22的表达，CCK-8法检测细胞存活率，划痕实验与Transwell小室法分别检测细胞的迁移与侵袭能力，Western blot法检测VEGF和P53蛋白表达。结果：卵巢癌组织中miRNA-22的水平显著低于正常卵巢组织；与control组相比，miRNA-22-m组细胞miRNA-22的表达显著增加，细胞存活率显著降低，细胞迁移数和侵袭数下降，VEGF和P53的蛋白表达水平显著下降。结论：miRNA-22的低表达可能与卵巢癌的发生发展有关。过表达miRNA-22能够抑制卵巢癌细胞株的增殖、迁移和侵袭，其机制可能与下调VEGF和P53的蛋白表达有关。