硬膜外腔注射蛇床子素对髓核致坐骨神经痛大鼠的疼痛行为学影响

摘要

目的:观察硬膜外腔注射蛇床子素对髓核致坐骨神经痛大鼠的疼痛行为学影响,并与临床常用药物得宝松、左旋布比卡因的镇痛行为学效应比较.方法:雄性SD (Sprague Dawley)大鼠48只,制作髓核致坐骨神经痛大鼠模型,随机分为6组(每组8只):手术模型组(G1)、生理盐水组(G2)、DMSO(二甲亚砜,dimethyl sulfoxide)组(G3)、蛇床子素组(G4)、左旋布比卡因组(G5)、得宝松组(G6),除了G1组不给任何处理,其他五组分别在术后第3天经硬膜外腔注射生理盐水、10％DMSO、2％蛇床子素、0.25％左旋布比卡因、0.3 mg/kg得宝松各50μl.检测大鼠术前1d,术后第1d、3d、7d、14d、21d及给药后1h、4h、8h、24 h、48 h的疼痛行为学指标(50％机械痛缩足阈值和热痛缩足潜伏期).结果:G1组的50％机械痛缩足阈值和热痛缩足潜伏期在术后第7天达到最低值(P＜0.05)；2％蛇床子素和0.3 mg/kg得宝松各50μl分别与溶剂生理盐水、10％DMSO和0.25％左旋布比卡因50μl比较,可持久(21天)提高50％机械痛缩足阈值和热痛缩足潜伏期(P＜0.05)；0.25％左旋布比卡因50 μl提高疼痛行为学指标的作用比2％蛇床子素和0.3mg/kg得宝松弱(P＜0.05)；术后第7d2％蛇床子素提高疼痛行为学指标的作用比0.3 mg/kg得宝松弱(P＜0.05),而术后第21 d 2％蛇床子素提高热痛缩足潜伏期作用比0.3 mg/kg得宝松强(P＜0.05).结论:2％蛇床子素50 μl硬膜外腔注射可有效改善髓核致坐骨神经痛大鼠的疼痛行为学反应,其效应与得宝松比较有差异,但比左旋布比卡因强.%Objective: To observe the effects of epidural injection of Osthole on the pain-induced behaviors in rats with nucleus pulposus-induced sciatica and compare it with other clinical agents (Diprospan and Levobupivacaine). Methods: 48 male SD (Sprague Dawley) rats were selected and made into nucleus pulposus-induced sciatica models. The rats were randomly and evenly divided into 6 groups (8 rats per group), surgical model group (G1), normal saline group (G2), 10% DMSO (dimethyl sulfoxide) group (G3), Osthole group (G4), Levobupivacaine group (G5) and Diprospan group (G6). Group Gl was not treated; the other 5 groups were respectively administrated with normal saline, DMSO (10%), osthole (2%), Levobupivacaine (0.25%), Diprospan (0.3 mg/kg) (50 |il for each agent) by epidural injection on the 3 rd day after operations. The pain-induced behavioral responses (50% paw withdrawal mechanical threshold and paw withdrawal thermal latency) were measured 1 d, 3 d, 7 d, 14 d, 21 d after operations and 1 h, 4 h, 8 h, 24 h, 48 h after drug administration. Results: 50% paw withdrawal mechanical threshold and paw withdrawal thermal latency in Gl reached the lowest value at 7 d after operations (P < 0.05).rnCompared with normal saline, 10%DMSO, 0.25% Levobupivacaine, Osthole (2%) and Diprospan (0.3 mg/kg) (50 μl for each agent) had persistent increasing effects (21 d) on 50% paw withdrawal mechanical threshold and paw withdrawal thermal latency (P < 0.05); 50 μl Levobupivacaine had weaker increasing effects on pain-induced behavioral responses than Osthole (2%) and Diprospan (0.3mg/kg, P < 0.05); On postoperative day 7, osthole (2%) had weaker effects on pain-induced behavioral responses than Diprospan (0.3mg/kg, P < 0.05); But on postoperative day 21 Diprospan (0.3 mg/kg) had weaker effects on paw withdrawal thermal latency than Osthole (2%). Conclusion: Epidural injection of Osthole (2%) has a good efficacy in improving the pain-induced behaviors in rats with nucleus pulposus-induced sciatica. The effect is different from Diprospan but stronger than Levobupivacaine.