大豆共生结瘤相关性状QTL定位信息整合及候选基因分析

摘要

Symbiotic nodulation in soybean is an important agronomic trait affecting soybean biological nitrogen fixation. Few QTLs for symbiotic nodulation with small confidence interval could be applied in practice. Our pur-pose was to integrate the results of previous studies and propose meta - QTL. Meta - analysis was carried out based on 48 QTLs about symbiotic nodule - related traits across 4 independent studies,and two meta - QTLs controlling nodule number,nodule size and nodule dry - weight were proposed. Six candidate genes were screened from the in-terval of meta - QTL and their expressional patterns were analyzed by qRT - PCR in cultivar soybean and wild soy-bean inoculated with rhizobia. Among them,4 genes may be involved in symbiotic nodulation in soybean. This two meta - QTLs should prove helpful in identification and functionality study of candidate genes associated with symbi-otic nodule - related traits.%目前基于大豆共生结瘤QTL定位的结果少、置信区间大,难以应用于实践中,因此整合前人研究结果、缩短置信区间具有重要意义.本研究针对大豆共生结瘤相关性状,搜集国内外已报道的48个QTL定位结果,利用Meta分析得到2个分别控制结瘤数目、结瘤大小和结瘤干鲜重的Meta-QTL.在区间内选择候选基因,利用野生大豆和栽培大豆验证候选基因在接种根瘤菌后的表达模式,明确候选基因与共生结瘤的关系.对Meta-QTL区间内的6个候选基因进行qRT-PCR检测及初步生物信息学分析,结果表明其中4个基因有可能为大豆共生结瘤性状的候选基因.本研究的实验方法对QTL定位区间内候选基因的确定和功能研究具有指导意义.